Will cerium dioxide accelerate wound healing?
Cells that heal wounds will be able to grow faster
A group of Russian scientists has developed an inexpensive method for accelerating fibroblast growth using cerium dioxide CeO 2 nanoparticles.
One of the problems of cell therapy is that some types of cells are difficult and long to cultivate in the laboratory. To enhance cell division, expensive drugs are used, which makes treatment inaccessible. Scientists from the Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences (ITEB RAS) in Pushchino, led by Anton Popov, have developed a method to accelerate cell cultivation.
The object of the study was fibroblasts – connective tissue cells that play an important role in wound healing. To activate their growth, scientists used a colloidal solution (sol) of cerium dioxide nanoparticles. The solution was added to the nutrient medium for cultures of primary fibroblasts taken from mouse embryos.
"When growing cells in vitro, that is, outside the body, not optimal conditions are created. Under in vitro conditions, the oxygen level is increased compared to in vivo conditions, inside the body. This affects the metabolism of cells: they develop oxidative stress. The addition of SeO 2 nanoparticles reduces the level of oxidative stress, simulating normal growth conditions in the body. Stem cell culture begins to grow faster," Anton Popov, the head of the research, a junior researcher at the Laboratory of Cell and Tissue Growth at ITEB RAS, explained the main idea of the study.
The results of the study on mouse fibroblasts were published back in May in the journal Materials Science and Engineering with. "In mice, we showed for the first time that these molecular mechanisms are possible at all," Popov said.
Researchers are currently working on creating substrates with cerium dioxide nanoparticles for cell culture. "We want to make substrates into which nanoparticles will be integrated, but they will not be able to get directly into the cell. This will remove questions during clinical trials about exactly where CeO 2 will go. In our case, nothing foreign, inorganic appears in the cell," Popov explained.
The study of Popov's group is especially relevant due to the fact that on January 1, the law on biomedical cell products came into force in Russia, which allows the use of living cells for therapy. Biomedical cellular products (BMCP) are fundamentally different from both medicines and medical devices. To obtain BMCP from the donor's body, a piece of biomaterial with a size of several millimeters is taken.
"During the production process, cells are scaled, modified, combined with some other drugs, and a new quality appears in the production process - a biomedical cell product. Taking into account modern technological capabilities, neurons or heart cells can be obtained from a small biopsy (material obtained by biopsy) of the skin. The possibilities here become limitless," said Andrey Vasiliev, one of the developers of the law, director of the N.K. Koltsov Institute of Developmental Biology of the Russian Academy of Sciences.
The paper, which describes the effect of SeO 2 nanoparticles on human cells, is published in the journal Nanomechanics Science and Technology: An International Journal.
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24.01.2017